SURG-01. DEVELOPMENT OF MURINE MODEL OF LASER INTERSTITIAL THERMOTHERAPY (LITT) FOR BRAIN TUMORS

نویسندگان

چکیده

Abstract The objective of this study was to develop and test a murine system for delivery laser interstitial thermotherapy (LITT). A built that allows stereotactic placement the into mouse brain real-time thermometry with custom-made thermometer holder. Tissue heating curves Laserglow Technologies 1064nm experiments testing tissue were performed by placing in temperature monitoring via thermal probe. adapted use frame utilizing Hamilton syringe holder consistent precise C57BL/6 mice samples analyzed H&E staining. First, ideal output established using chicken breast. Laser dial setting 4.38 successfully achieved slow while 4.43 4.50 accomplished it rapidly, reaching ablative 43C three half minutes. Next, revealing probe would require separate measurements heating. designed fashioned trigonometric calculations allowed within 3mm spacing during ablation. Mice anesthetized treated ablation at 4.43. staining demonstrated clear evidence localized identifiable visible lesions on slides. successful development acquisition data coupled live model LITT proves our protocol is ready research therapeutic combinatorial effects tumor bearing mice.

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ژورنال

عنوان ژورنال: Neuro-oncology

سال: 2022

ISSN: ['1523-5866', '1522-8517']

DOI: https://doi.org/10.1093/neuonc/noac209.967